The role of chlorophyll in photosynthesis is irreplaceable and an important indicator of plant growth and physiological changes. The determination of its content is influenced by many external factors such as light, temperature, oxygen and so on. The betel nut has a very large area in Hainan, China. Researching its chlorophyll content is very important for the cultivation of betel nut.
The chlorophyll content in leaves of Areca acuminata seedlings was determined. The effects of different sample weights, extraction time, solvents, light and high temperature on the determination of chlorophyll content were compared, and a mathematical model for the determination of chlorophyll content with different sample weights and extraction time was established. Provide reference for physiological and biochemical research of betel nut. These chlorophyll content determination methods can damage the health of the leaves and can be effectively measured using a chlorophyll content meter.
The content of betel leaf was determined by the traditional method for determining the content of chlorophyll. When the volume of extraction solvent is the same, there is a very significant negative correlation between the amount of weighed samples and the chlorophyll content. That is, the more samples are weighed, the smaller the chlorophyll content is. Comparing the relative contents of different chlorophylls, it is necessary to ensure that the sampling sites are the same, the width of the blades is the same, and the sample size is not much different. However, the smaller the sample size, the greater the error. In this study, it is recommended to use a minimum sample weight of 0.2 g and add 20 ml of organic solvent.
The time of leaching was positively correlated with the chlorophyll content. The longer the storage time, the higher the chlorophyll content. However, after 26 hours, the chlorophyll content decreased. This may be due to the fact that after a period of time, chlorophyll has been extracted. Degradation. The chlorophyll content measured at high temperature and short time was higher than that at room temperature for a long time. The reason is that under the condition of not destroying chlorophyllase, high-temperature extraction of chlorophyll has a short time and high speed, and the damage to chlorophyll is reduced as much as possible, so the measured value is higher. Isolated chlorophyll is very sensitive to light, light and oxygen can cause irreversible decomposition of chlorophyll, so the chlorophyll value measured in dark treatment is higher than that seen in light treatment. The results of the study showed that the chlorophyll content measured under light-free treatment was close to the true value when extracted rapidly at high temperature for 2 hours.
If you want to avoid the errors caused by the sample preparation process and reduce the damage to the leaves, you can use the chlorophyll content analyzer to measure. This instrument can not only protect the leaves from damage, but also provide scientific and accurate data for the research work. Provide protection information.
The chlorophyll content in leaves of Areca acuminata seedlings was determined. The effects of different sample weights, extraction time, solvents, light and high temperature on the determination of chlorophyll content were compared, and a mathematical model for the determination of chlorophyll content with different sample weights and extraction time was established. Provide reference for physiological and biochemical research of betel nut. These chlorophyll content determination methods can damage the health of the leaves and can be effectively measured using a chlorophyll content meter.
The content of betel leaf was determined by the traditional method for determining the content of chlorophyll. When the volume of extraction solvent is the same, there is a very significant negative correlation between the amount of weighed samples and the chlorophyll content. That is, the more samples are weighed, the smaller the chlorophyll content is. Comparing the relative contents of different chlorophylls, it is necessary to ensure that the sampling sites are the same, the width of the blades is the same, and the sample size is not much different. However, the smaller the sample size, the greater the error. In this study, it is recommended to use a minimum sample weight of 0.2 g and add 20 ml of organic solvent.
The time of leaching was positively correlated with the chlorophyll content. The longer the storage time, the higher the chlorophyll content. However, after 26 hours, the chlorophyll content decreased. This may be due to the fact that after a period of time, chlorophyll has been extracted. Degradation. The chlorophyll content measured at high temperature and short time was higher than that at room temperature for a long time. The reason is that under the condition of not destroying chlorophyllase, high-temperature extraction of chlorophyll has a short time and high speed, and the damage to chlorophyll is reduced as much as possible, so the measured value is higher. Isolated chlorophyll is very sensitive to light, light and oxygen can cause irreversible decomposition of chlorophyll, so the chlorophyll value measured in dark treatment is higher than that seen in light treatment. The results of the study showed that the chlorophyll content measured under light-free treatment was close to the true value when extracted rapidly at high temperature for 2 hours.
If you want to avoid the errors caused by the sample preparation process and reduce the damage to the leaves, you can use the chlorophyll content analyzer to measure. This instrument can not only protect the leaves from damage, but also provide scientific and accurate data for the research work. Provide protection information.
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